(*AES-corresponding author)
Sikora AE*, Wierzbicki IH, Zielke RA, Ryner RF, Korotkov KV, Buchanan SK, Noinaj N. (2017) Structural and functional insights into the role of BamD and BamE within the β-barrel assembly machinery in Neisseria gonorrhoeae.J Biol Chem. 2017 Dec 11. pii: jbc.RA117.000437. doi: 10.1074/jbc.RA117.000437. [Epub ahead of print] PubMed PMID: 29229778.
Unemo M, Sikora AE. (2017)Infection: Proof of principle for effectiveness of a gonorrhoea vaccine.Nat Rev Urol. 2017 Nov;14(11):643-644. doi: 10.1038/nrurol.2017.139. Epub 2017 Aug 31. PubMed PMID: 28858332; PubMed Central PMCID: PMC5735823.
Liu Y, Hammer LA, Liu W, Hobbs MM, Zielke RA, Sikora AE, Jerse AE, Egilmez NK, Russell MW. (2017) Experimental vaccine induces Th1-driven immune responses and resistance to Neisseria gonorrhoeae infection in a murine model.Mucosal Immunol. 2017 Nov;10(6):1594-1608. doi: 10.1038/mi.2017.11. Epub 2017 Mar 1. PubMed PMID: 28272393; PubMed Central PMCID: PMC5591041.
Dugovich BS, Peel MJ, Palmer AL, Zielke RA, Sikora AE, Beechler BR, Jolles AE, Epps CW, Dolan BP. (2017) Detection of bacterial-reactive natural IgM antibodies in desert bighorn sheep populations. PLoS One. 2017 Jun 29;12(6):e0180415. doi: 10.1371/journal.pone.0180415. eCollection 2017.
#Sikora AE*, Mills RH, Weber JV, Hamza A, Passow BW, Romaine A, Williamson ZA, Reed RW, Zielke RA, Korotkov KV.(2017) Peptide Inhibitors Targeting the Neisseria gonorrhoeae Pivotal Anaerobic Respiration Factor AniA.Antimicrob Agents Chemother. 2017 Jul 25;61(8). pii: e00186-17. doi: 10.1128/AAC.00186-17. Print 2017 Aug. PubMed PMID: 28584144; PubMed Central PMCID: PMC5527597.
#This manuscript was featured: http://advantage.oregonstate.edu/feature-story/stop-sexually-transmitted-superbug-aleksandra-sikora-took-unconventional-approach
#Baarda BI, Emerson S, Proteau PJ, Sikora AE*. Deciphering function of new gonococcal vaccine antigens using phenotypic microarrays.(2017) J Bacteriol. 2017 Jun 19. pii: JB.00037-17. doi: 10.1128/JB.00037-17. [Epub ahead of print] PubMed PMID: 28630127; PubMed Central PMCID: PMC5553031
#Manuscript selected as Editor’s Highlight “Articles of significant interest”: http://jb.asm.org/content/199/17/e00423-17.full
#Media coverage:
http://klcc.org/post/osu-research-promising-basis-gonorrhea-vaccine
https://medicalxpress.com/news/2017-09-groundbreaking-effort-gonorrhea-vaccine-candidates.html
http://news.xinhuanet.com/english/2017-09/20/c_136622500.htm
http://www.opb.org/news/article/oregon-state-university-gonorrhea-vaccine-research/
http://ijpr.org/post/osu-team-identifies-proteins-could-lead-gonorrhea-vaccine#stream/0
http://www.sciencenewsline.com/news/2017092015280055.html
https://micronow.org/science/two-cell-envelope-proteins-may-be-good-gonorrhea-vaccine-candidates/
http://www.ktvz.com/news/osu-researchers-identify-gonorrhea-vaccine-candidates/623737139
http://cheerioweb.com/gonorrhea-vaccine-key-discovery-raises-hope-for-treatment.html
http://china.org.cn/world/Off_the_Wire/2017-09/20/content_41616224.htm
Wierzbicki IH, Zielke RA, Korotkov KV, Sikora AE. Functional and structural studies on the Neisseria gonorrhoeae GmhA, the first enzyme in the glycero-manno-heptose biosynthesis pathways, demonstrate a critical role in lipooligosaccharide synthesis and gonococcal viability. 2017 Apr;6(2). doi: 10.1002/mbo3.432. Epub 2017 Jan 7. PubMed PMID: 28063198; PubMed Central PMCID: PMC5387315.
Unemo, M., Golparian, D., Sánchez-Busó, L., Grad, Y., Jacobsson, S., Ohnishi, M., Lahra, M., Limnios, A., Sikora, A.E., Wi, T., Harris, S. (2016). The novel 2016 WHO Neisseria gonorrhoeae reference strains for global quality assurance of laboratory investigations – phenotypic, genetic and reference genome characterization. J Antimicrob Chemother. 2016 Nov;71(11):3096-3108. Epub 2016 Jul 17.
Zielke, R.A., Wierzbicki, I.H., Baarda, B.I., Gafken, P.R., Soge, O.O., Holmes, K.K., Jerse, A.E., Unemo, M. and Sikora, A.E.* (2016). Proteomics-driven antigen discovery for development of vaccines against gonorrhea. Molecular & Cellular Proteomics. 2016 May 2. pii: mcp.M116.058800.
Bonventre, J.A., Zielke, R.A., Korotkov, K.V and Sikora, A.E.* (2016). High-throughput screening campaign to identify small molecule compounds targeting essential bacterial Obg proteins. PloSOne. 2016 Feb 5;11(2):e0148222. doi: 10.1371/journal.pone.0148222. eCollection 2016
Baarda, B.I., and Sikora, A.E.* (2015). Proteomics of Neisseria gonorrhoeae: the treasure hunt for countermeasures against an old disease. Frontiers in Microbiology. 2015 Oct 26. 6:1190. doi: 10.3389/fmicb.2015.01190
Zielke, R. A., Wierzbicki, I. H, Baarda, B.I., and Sikora, A.E.* (2015). The Neisseria gonorrhoeae Obg protein is an essential ribosome-associated GTPase and a potential drug target. BMC Microbiology. 2015 Jun 30;15(1):129. doi: 10.1186/s12866-015-0453-1
Park, B.R., Zielke, R.A., Wierzbicki, I.H., Mitchell, K.C., Withey, J.H., and Sikora, A.E.* (2015). A metalloprotease secreted by the type II secretion system links Vibrio cholerae with collagen. Journal of Bacteriology. 2015 Mar 15;197(6):1051-64. doi: 10.1128/JB.02329-14.
Zielke, R.A., Gafken, P.R., and Sikora, A.E.*(2014). Quantitative Proteomic Analysis of the Cell Envelopes and Native Membrane Vesicles Derived from Gram-Negative Bacteria. Current Protocols in Microbiology. 2014. Aug 1;34:1F.3.1-1F.3.16. doi: 10.1002/9780471729259.mc01f03s34.
Zielke, R.A., and Sikora, A.E.* (2014). Isolation of Cell Envelopes and Naturally Released Membrane Vesicles of Neisseria gonorrhoeae. Current Protocols in Microbiology. 2014 Aug 1;34:4A.3. 17. doi: 10.1002/9780471729259.mc04a03s34
Zielke, R.A., Simmons, R.S., Park, B.R., Nonogaki, M., Emerson, S., and Sikora, A.E*. (2014). The type II secretion pathway in Vibrio cholerae is characterized by growth-phase dependent expression of exoprotein genes and is positively regulated by sigmaE. Infection and Immunity. 2014 Jul;82(7):2788-801. doi: 10.1128/IAI.01292-13. Epub 2014 Apr 14.
#Zielke, R.A., Wierzbicki, I.H., Weber, J.V., Gafken, P.R., and Sikora, A.E.*(2014). Quantitative proteomics of the Neisseria gonorrhoeae cell envelope and membrane vesicles for the discovery of potential therapeutic targets. Molecular and Cellular Proteomics. 2014;13(5):1299-317.
# This manuscript was featured in: Newsletter of The Alliance for the Prudent Use of Antibiotics (Volume 31, No 1); by the American Society for Biochemistry and Molecular Biology blog in April, 2014 (http://wildtypes.asbmb.org/2014/04/09/finding-new-targets-for-attack-on-the-bacterium-that-causes-gonorrhea/), as well as Global Medical Discovery in July, 2014. (https://globalmedicaldiscovery.com/key-scientific-articles/quantitative-proteomics-of-the-neisseria-gonorrhoeae-cell-envelope-and-membrane-vesicles-for-the-discovery-of-potential-therapeutic-targets/).
As well as highlighted by the local media (TV-KEZI http://www.kezi.com/breakthrough-osu-gonorrhea-study/ and newspapers:
http://www.dailybarometer.com/news/gonorrhea-on-the-verge-of-drug-resistance/article_c1752108-ba14-11e3-a7eb-001a4bcf6878.html
http://www.vocativ.com/culture/health-culture/soon-cure-super-gonorrhea/
http://www.ziptrials.us/sexual-health/std/new-breakthrough-in-gonorrhea-treatment/
http://www.firstwordpharma.com/node/1199696#axzz3Napc4Cen
- Sheidy DT, Zielke RA. Analysis and expansion of the Escherichia coli protein ProQ. PLoSOne 2013 Oct 25;8(10):e79656. doi: 10.1371/journal.pone.0079656. eCollection 2013.
- Tran, N., Zielke, R.A., Vining, O. B., Azevedo, M.D., Armstrong, D.J., Banowetz, G.M., McPhail, K.L and Sikora, A.E.*(2013). Development of a quantitative assay amenable for high-throughput screening to target the type II secretion system for new treatments against plant-pathogenic bacteria. Journal of Biomolecular Screening. 1087057113485426.
- Sikora, A.E.*(2013). Proteins secreted via the type II secretion system: Smart strategies of Vibrio cholerae to maintain fitness in different ecological niches. PLoS Pathogens, 2013 Feb;9(2):e1003126.
- Johnson, T.L., Sikora, A.E., Zielke, R. A. and Maria Sandkvist (2013). Fluorescence microscopy and proteomics to investigate subcellular localization, assembly and function of the type II secretion system. The Bacterial Cell Surface: Methods and Protocols. 2013;966:157-72.
- Sikorska, J., Parker-Nance, S., Davies-Coleman, M. T., Vining, O. B., Sikora, A. E., McPhail, K. L. (2012). Antimicrobial rubrolides from a South African species of Synoicum tunicate. Journal of Natural Products, 2012, Oct 26: 75(10):1824-7.
- Sikora, A.E., Zielke, R. A., Lawrence, D. A., Andrews, P. C., Sandkvist M. (2011). A comprehensive proteomic analysis of Vibrio cholerae type II secretome reveals new proteins including three related serine proteases. Journal of Biological Chemistry, 2011 May 13; 286(19):16555-66.
- Sikora, A. E., Beyhan, S., Bagdasarian, M., Yildiz, F. H., Sandkvist M. (2009). Cell envelope perturbation induces oxidative stress and changes in iron homeostasis in Vibrio cholerae. Journal of Bacteriology,2009 Sep; 191(17):5398-408
- #Lybarger, S.R., Johnson, T.L., Gray, M. D, Sikora, A.E., Sandkvist, M. (2009). Docking and assembly of the type II secretion system of Vibrio cholerae. Journal of Bacteriology, 2009 May; 191(9):3149-61.
#Manuscript selected in Microbe Editor’s Spotlight, June 2009.
- Sikora AE, Zielke R, Wegrzyn A, Wegrzyn G. DNA replication defect in the Escherichia coli cgtA(ts) mutant arising from reduced DnaA levels. (2006). Arch. Microbiol. 2006 Jun;185(5):340-7.
- Sikora AE, Zielke R, Datta K, Maddock JR. The Vibrio harveyi GTPase CgtAV is essential and is associated with the 50S ribosomal subunit. (2006). Journal of Bacteriol. 2006 Feb;188(3):1205-10.
- Sikora AE, Datta K, Maddock JR. Biochemical properties of the Vibrio harveyi CgtAV GTPase. (2006) Biochem. Biophys. Res. Commun. 2006 Jan 27;339(4):1165-70.
- Ulanowska K, Sikora A, Wegrzyn G, Czyz A. (2003) Role of the cgtA gene function in DNA replication of extrachromosomal elements in Escherichia coli. (2003). Plasmid. 2003 Jul;50(1):45-52.
- Zielke R, Sikora A, Dutkiewicz R, Wegrzyn G, Czyz A. Involvement of the cgtA gene function in stimulation of DNA repair in Escherichia coli and Vibrio harveyi.(2003). Microbiology. 2003 Jul;149(Pt 7):1763-70.
