Finishing Fieldwork in the Intertidal

Last weekend I concluded my fieldwork for both Otter Rock and Cascade Head 2018 intertidal monitoring for ODFW. On Saturday we finished community mussel bed surveys that examined the intertidal community for potential changes from reduced populations of sea stars caused by wasting in 2014. For this we meet at Otter Rock at 5:20am while it was still pitch black outside. We proceeded to walk to the site in the dark using the light from a few headlamps and phones.

Heading to site at Otter Rock before dawn

For this survey we measured the height and depth of the mussel bed, abundance of mussel predators (sea stars and whelks), and counted mussel recruits. We count the whelks because they may take over the role of controlling the lower limit of the mussel bed. This is normally the role of sea stars (especially Pisaster ochraceus) but their populations are significantly reduced due to wasting.

Counting mussel recruits at Otter Rock 

Whelk: predator of mussels

On Sunday I woke up before sunrise again. At 5am I was on my way to Cascade Head to complete the last sea star wasting survey of 2018. And good news was that we saw very little wasting sea stars! Unfortunately though three of our transects that we sample were under water even with the -1.9 tide. In previous months and years there had been more sand that made the pools shallower allowing for ODFW to count sea stars in those areas but this year the sand was all washed away and we were not able to sample them. We were still able to survey around 200 sea stars during the belt transect and over 200 sea stars for the timed searches.

Timed search surveys at Cascade Head

 

Timed search survey at Cascade Head

Once I finished the fieldwork I had to enter my data and analyze it before the final presentations we gave on Friday. It lead to a couple of very busy days but I was able to finish everything. For those of you who were unable to come to the final presentations I will give a brief summary of what I found. The biggest conclusion was that sea stars are very healthy! Since 2015 when monitoring began the percentage of sea stars showing signs of wasting has deceased to very minimal levels (below 2%) at both sites by 2018. The other big conclusion I determined was that population of sea stars remained similar to 2015 over the four years based on densities. There was some fluctuation but it was not statistically significant at Cascade Head and for Otter Rock it was only one year having slightly higher densities but then it dropped back down the next year. There are two main possibilities to explain why we aren’t seeing an increase in densities over the four years. One is that by 2015 populations had already recovered from sea star wasting. The other explanation is that four years is not enough time to see recovery and it will take more years of monitoring to observe an increase in sea star density indicating that they have recovered.

For those of you who came to the final presentations, thank you for your support. And for those who are reading and were unable to come, I hope you enjoyed learning about my project and you can check out a picture of my poster below:

Presenting my poster

 

Final Results of the Mesocosms

In my last blog post, I introduced you to the excitement Newport offers outside of Hatfield Marine Science Center, but left you hanging on how the mesocosm project turned out.

We planned on running experiments three days in a row, leaving the mesocosms out in the field for the entire duration. We needed to collect over 360 juvenile Dungeness crab and over 18 Pacific staghorn sculpin, which proved harder than we expected. We quickly saw that we would need to adjust our study due to the natural progression of second instars growing into fourth or fifth instar crabs. The crabs were too large for the Pacific staghorn sculpins to eat, making it difficult to run a predation experiment. However, we realized that the large crab size could be a benefit as it would allow us to study the crabs’ behavior without any predation while also reducing a factor of loss when retrieving the crabs. This increase in crab carapace allowed us to reduce the number of crabs needed to 10 per mesocosm instead of 20, another benefit. We spent three days beach seining at low tide and setting minnow traps overnight to collect the necessary number of organisms.

Brett Dumbauld of USDA-ARS beach seining for juvenile Dungeness crab and Pacific staghorn sculpin in Yaquina Bay, OR.

Water tables in the EPA lab housing over 250 juvenile Dungeness crab and over 40 Pacific staghorn sculpin.

With all of the pieces together, we were able to move forward and set-up the mesocosms in the field to begin running experiments. The mesocosms were set the same as they had been during the first trial, each containing different combinations of two habitat types (on-bottom oyster aquaculture, eelgrass, open mud) in three controls and duplicated in three treatments.

An example of a mesocosm set-up. One side contains eelgrass, the other oysters placed to mimic on-ground oyster aquaculture.

We then prepared the Pacific staghorn sculpins by starving them for 24 hours before they were put in the field. We had previously decided that we would experiment with different lengths of time that the crabs were exposed to the sculpin to see if it had any effect on their behavior. We decided to begin one trial when the water was low enough that it wouldn’t be spilling over the top of the mesocosms (about 2.5′). This trial was run for 2 hours, wherein predators were left in the mesocosms. We then reset the trial by removing and counting predators and prey before adding more organisms for a 24-hour trial which we would come back to the next morning. As we approached the mesocosms that morning with the water just around the tops, we noticed them rocking back and forth.

NOOOO! How were we going to run our 24-hour experiment without the crabs and sculpins escaping? We ran back to Hatfield during our 2-hour wait period and brought back a drill and rebar to reinforce the mesocosms, hoping it would do. Since we already had the organisms prepared, it was best to run the 24-hour experiment and just see what would happen.

Kelly Muething and Anna Bolm clearing out the different habitats after a 24-hour habitat selection experiment involving juvenile Dungeness crab and Pacific staghorn sculpin, in Yaquina Bay, OR.

We had some pretty interesting results. In the 2-hour experiment, we retrieved 95% of the crabs while in the 24-hour experiment we retrieved 106% of the crabs. This was the opposite of what we expected since the mesocosms had been rocking, but apparently some other crabs had run in rather than escape. Given that we only ran two trials, we can’t conclude any real results, but did see some patterns. Crabs preferred oyster shell over both eelgrass and open mud, whether or not there was a predator. The sculpins’ presence didn’t seem to have much impact on crab habitat selection, possibly because they had outgrown the sculpins’ ability to prey. All in all, the mesocosms were a success and Brett plans on using them again next summer, earlier in the crab season to test the second instars.

Last Friday, I presented my work and then participated in a poster session, a really rewarding experience. It felt good to share what I had been working on and I appreciated the exercise of thinking about how to communicate the project to others. It was also informative to see what the other Sea Grant scholars had been working on as well as converse with scientists about our work.

Poster shown on the mesocosms at Oregon Sea Grant poster session.

It’s been a really incredible summer living and working at Hatfield Marine Science Center. I am very grateful to have been given this opportunity and feel lucky to have had such wonderful mentors to work with. To celebrate the end of the summer and completing the final presentation and poster session, my husband guided me out on my first sea kayaking trip, exploring the sea caves beneath Cascade Head. Rising and falling with the swell is an incredible feeling, the water looking like hills around you. We watched a whale play about 100 meters away before heading into a cave. I have to say, it was pretty scary and amazing at the same time. Paddling into darkness with waves booming around you would spook anyone, right? It was cool seeing all of the birds nesting along the rock cliffs, Pacific sea nettles swimming around, and sea stars and anemone exposed at low tide. We also spotted some floating tubes which turned out to be squid eggs. All in all, the perfect end to a perfect summer and a reminder of how much we love the area. We’re hoping to move to Newport so I can continue volunteering and learning at Hatfield while looking for work.

An Adventure to Central and Eastern Oregon

I still can’t believe that I only have two more week until my final presentation and three weeks left in total.

The first couple of weeks, I spent a lot of time researching the Endangered Species Act (ESA) as mentioned in my earlier blogs. I had questions which I did not have a solid answers too. The questions I faced was, what happens when two listed species overlap. An example of this is the Southern Resident Killer Whales (SRKW) and the Chinook salmon. Last week a SRKW gave birth, unfortunately the newborn did not make it. Since then, the mother still carries the dead newborn on its nose as it migrates with the pod. We don’t know how long this will last, and how much stress this puts on the mother. Unlike other killer whales, SRKW feed primarily on Chinook salmon. From what is happening with the SRKW, people are raising question to provide food for the SRKW. Well, what does this mean? This mean hatcheries will have to crank up Chinook production. However, there is a downside to increasing salmon production. Increasing hatcheries production may increase risk towards Chinook salmon which are also listed. This is a challenging question to answer when prioritizing one species over the other could bring one specie to extinction which defeats the purpose of the ESA. This situation becomes increasing complex given we have very little information on the populations dynamics and rapid changes in climate altering ecosystems.

This week I went down to Bend with Wesley, another Oregon Sea Grant Summer Scholar. We did a six hours hike up to Broken Top. The first portion of the hike was great because we had a huge cloud hovering over us as we climb up in elevation. Luckily the sun was not beating down on us. There was a part in the hike which was very sketchy. The path was very narrow and the gravel was unstable, not to mention there was some snow and nothing for us to grip on with our hands as we slowly move across. Aside from the scary part of the hike. The view was fantastic! Broken top in front, Three Sisters Mountain, Mt. Bachelors and many more. We kept hiking until we reached the lake at the bottom of Broken Top. The lake had no name which is why I think they named it No Name Lake. By the time we had reached the lake we were exhausted, and decided to dip into the lake before heading back to the trail head. I planned on hiking Crater Lake the next morning but after this hike, my legs had enough. Crater Lake would have to wait another day.

On our way back to Portland, we took a detour towards Warm Springs. I heard there was a hot springs there and I wanted to see the Eastern parts of Oregon. The Eastern part of Oregon was of course drier, however the landscape was very nice. Before we reached the hot springs we encountered wild horses. They were grazing along the side of the road and blocking the road. I’ve never seen a wild horse. They were well groomed and their colors varied unlike the domesticated horses I’ve seen. We finally reached the hot springs without running any wild horse over. The hot spring was not what I had in mind. It was a swimming pool with two slides similar to a water park. We came all this way, so I had to get into the water and at least slide down the slides. It was 30 feet high. I wanted to get a thrill in before we hit the road again. Until next blog, that is all I have for now.

Delving into Human Dimension Research

At the end of my last blog post, I left you hanging with what is human dimension research and how does it play into my work this summer. If you haven’t looked up the definition in the last two weeks, or read blog posts by my fellow Summer Scholars,human dimension research examines how we interact with and utilize the environment around us. This information is commonly gathered through surveying the general populous, as well as specific interest groups. For instance, Oregon Department of Fish and Wildlife has conducted surveys at various marine reserves to understand how who is visiting these areas, in addition to their overall experience while at the reserve. When looking at this “who?” question, surveyors may look at everything from education level to income to ethnicity to what city exactly the person in question lives. In order for a natural area to garner more attention, and in turn building up public’s desire to protect nature, surveyors may also question what can be done better. Sometimes it is providing more facilities, such as campgrounds or hotels at which travelers can stay, or picnic tables where families can eat their lunch. In other instances, it may be offering more educational programs so guests understand the value of the refuge, reserve, park, etc…

 

In my case, Haystack Rock Awareness Program (HRAP) has been looking to gain a better understanding as to where our visitors come from and if they both understand and value this unique place. While the organization has collected a great deal of data for over fifteen years, including the number of people we talk to during a shift and the number of people walking on the rocks instead of the sand, they have never delved into the background of these visitors. Therefore, my human dimension research centered on a survey that asked our guests these questions. I utilized a platform called Survey123 by ArcGIS to create the survey, as with ArcGIS’s technology we were able to ask people to pinpoint exactly where they live. This was an important component to HRAP as we talk to people from all over the world, not to mention many people who have lived here in Cannon Beach for a long time.

 

Based on my interactions on the beach and speaking with staff members who have been with the organization ranging from a year and a half to over sixteen years, I knew that the survey needed to have a range of questions – some of which were directed more specifically towards locals and some which more highlighted the experience of tourists. I also knew that while some people had never visited Haystack Rock, we also have a large portion of visitors who have come back for many years. Given all of this information, I built the survey with a few distinctive sections in mind. Like many surveys, I began with demographic questions, the most important of which being where does the person live. Other questions in this section focused on their socioeconomic background, including gender, age, income, and ethnicity among other personal details. It is important to note that the person was free to skip any question which they did not feel comfortable answering.

 

The next section centered around the frequency with which the person visited Haystack Rock and what brought them to our reserve. Questions encompassed the number of times and how recently they had visited, how they first heard about us, and why they visited Haystack Rock. Obviously, the format of the question varied between local and tourist, but each addressed the same question – why do you visit the Rock? Within this portion of the survey, I also wanted to find out just how well known our organization was to those who had not been to Haystack Rock before. HRAP is constantly trying to spread its presence as we believe this is one of the ways we can help ensure the conservation of the Rock.

 

The final section moved a little more into the nitty-gritty scientific details. For one, we were curious as to whether or not the public understood that Haystack Rock is actually two different parts of the same whole according to US Fish and Wildlife Service. One part being the Marine Garden, or intertidal area littered with smaller rocks, while the other is the rock standing at 235 feet tall, which is part of the Oregon Islands Wildlife Refuge. Our theory when formulating the survey was that many people perceive our asking them not to stand on the rocks as HRAP’s personal rule, when in fact it is a federal regulation. To test this, a few questions were dedicated to the subject’s understanding of these regulations. The other half of the section asked whether education and enforcement needed to be improved/expanded at Haystack Rock as well as up and down the coast in other natural areas.

Can you tell what is the Marine Garden and what is the Wildlife Refuge based on this photo? Photo Credit: Haystack Rock Awareness Program

There is much more to the survey than what I have simplified it to in this blog post, so please check it out in the link below. Even better, if you have been to Haystack Rock, please take the survey! It only takes a few minutes and I am trying to get as many responses as possible in the next week. I am not including any of the results I have gathered to this point in this post because I don’t want it to skew anyone’s responses.

arcg.is/LybXv

On a completely unrelated note, I cannot believe I only have two weeks left here in Oregon before I return to the Sunshine State, but I have seen some incredible places and met some even more incredible people!

Otter Rock Surveys Complete for 2018

Since I have last posted, I successfully led and finished the last sea star wasting intertidal survey for Otter Rock Marine Reserve in 2018. We had a great turnout of volunteers and were able to survey everything very quickly. Unfortunately we did see a few sea stars with advanced signs of wasting. The good news was that the percent of sea stars wasting has continued to decrease at Otter Rock since the outbreak of the epidemic.

Ochre sea star showing signs of wasting

For this survey we identified, measured, and assigned disease codes to ochre sea stars, blood stars, and six-rayed sea stars along 5 established transects at Otter Rock. These transects are 5 meters long and we searched a meter to each side of the transect for a total of 10m2 for each transect. After we completed these types of surveys we conducted timed searches. For this, we record the length of time searched for and the number of people searching and then cover a large area looking for ochre sea stars and false ochre sea stars. When we find a sea star we record the species, size, and disease code. Currently I am analyzing the data from Otter Rock and looking forward to collecting the last of the data from Cascade Head Marine Reserve next weekend.

Otter Rock Intertidal

Oregon’s coast has a lot to offer other than its intertidal. Last weekend I went on a beautiful hike. The first part was called Hobbit Trail and I had to duck/crawl to hike through most of it. It was very cool to be surrounded by plants to the point where it felt like I was tunneling through the bushes and trees. The later part of the trail lead to Heceta Head Lighthouse. This trail was so different. It was right next to highway 101 but you would have no idea on the trail because the forest was amazing. The trees were very tall and the canopy dense so that it was very dark on the trail. Additionally there was a steep incline so that we entered the mist that was hovering above. This eerie setting made for great pictures and an excellent hike.

Hike to Heteca Head Lighthouse

Another great experience I have had was SMURFing this week. SMURF is an acronym for standard monitoring unit for the recruitment of reef fishes. These contraptions that are meant to simulate kelp environment are to capture juvenile fishes. In order to do this, a SMURF is attached to a mooring line by snorkelers and then recovered a couple of weeks later. We pull up next to the mooring in the boat and jump over the side, snorkel to the mooring, and wrap the SMURF with a large fine mesh net. A new SMURF is attached while the old one is carried back to the boat. Once we are back on the boat we shake out the contents of the SMURF and look for juvenile fish. This last week we followed this procedure for the SMURFs inside and outside Otter Rock Marine Reserve. During this time of year we don’t usually observe as many juvenile fish as this time we only got 8 juvenile rockfish. While sampling we saw whales, seals, and many birds. It was a great way to start the day.

 

OSG Summer 2.0: Interviewing Fishers along the Oregon Coast

On Thursday, July 12th, my mentor Dr. Beth Marino and I joined a virtual meeting. I had high hopes; for four weeks I had been waiting to hear if I had approval to conduct my primary research project, which was to interview fishermen up and down the Oregon coast. The work I had been doing up until this point was constructive for my own understanding of coastal attitudes and was applicable to the broader goals of the Human Dimensions Project of the ODFW Marine Reserves Program, but it didn’t feel like something I could own. Granted, the results of this well-being survey, which no doubt I will outline in my final blog post in a couple of weeks, fascinate me because they get to the root of how people think. They reveal the lenses by which people view the world, and the thought processes they engage in when confronted with change. I invested the time in making sense of these responses, but I was not involved in the initial process of helping those responses emerge.

This distinction is important to me because other than being a scientist, I am also an artist, a dancer. Creating and leaving my own mark on the world is a part of my character. This is why I was itching to get started on what I was brought to Oregon to do in the first place: to help stories be heard.

On Thursday, July 12th, we were virtually meeting with a member of Oregon State University’s Institutional Review Board who would decide if the revisions associated with my involvement in Beth’s project could adequately protect the confidentiality of the interviewees. Already a week delayed, we thought this would be the day. But not quite.

I had an interview lined up for the next day that I had to reschedule (which, in retrospect, was for the better–I wasn’t prepared for a daylong road-trip, despite my eagerness).

So I waited until Monday while Beth meticulously worked at getting the project revision approved. Noon ticked by, and I still hadn’t heard. One fisherman was ready to meet 45 minutes away, and I was just waiting to have the go ahead.

I got the text message at 4 pm. And the rest of my summer began.

Me with my trusty state-owned Ford Fusion, which has helped me travel approximately 200 miles up and down the coast to conduct interviews.

 

 

 

 

 

 

 

Without hesitation I gathered my recording equipment, hopped into the state car, and was on my way to Depoe Bay. My first interview exceeded all expectations: the fisherman I spoke with was very open about his responses, could see the world from multiple perspectives, and had a rich understanding of both his community and the biological world that his work depends upon. We had conversations about the marine reserves, management practices, conservation, and his life as a fisher…all of which lasted for 1 hour and 40 minutes (for perspective, we anticipate good interviews to last anywhere from 30 minutes to 2 hours). I drove back home beaming, for I felt like this was the type of work I was meant to do.

The next interview was two days later and 80 miles north of Newport in a beautiful place called Garibaldi. To hear my first impression and thoughts right after rolling up to the coffee shop, watch this video.  Garibaldi is situated in a beautiful slice of the Oregon coast right where the ocean pours into a freshwater valley. The neighboring town is Tillamook, famous for their dairy products, and while driving back I got the chance to briefly check out what the town is so famous for.

The Great Northern Railway stationed in Garibaldi, right outside of the coffee shop where I conducted my interview, with a smokestack in the background.

 

 

 

 

 

 

 

 

 

 

My second interview was entirely different from the first; this fisherman was a fourth generation fisher, and his sons and grandchildren are continuing the culture. His operation runs from Alaska to California and they catch everything from salmon to Dungeness crab. This hour and 20 minute long conversation, which touched on the same themes as before, went in entirely different directions–especially with respect to conservation and management. Being involved in multiple states, he noted that he felt a difference in how management and policy-making decisions were handled in Alaska versus Oregon. Though the “Oregon Way,” or the culture of public inclusion in government decision-making, is perceived as prevalent in Oregon, this fisher suggested that based on the model of Alaska there is room for improvement. He wished managers had more of an open door to those involved in commercial resource industries.

These interviews are intended to measure the impacts of the marine reserves on people in the commercial and charter fishing community, but this point illustrates how these conversations can be applied to issues beyond the marine reserves. They aim to represent a voice not typically heard, and so long as they are representative of the fishing community as a whole, these words can be used to inform management practices and policy. Local knowledge from fishers about the ocean itself can help scientists design more effective studies.  These conversations can open the door to more constructive dialogues about how we as humans relate to our environment.

Some fantastic rock formations in Tillamook Bay, captured while standing next to the railroad tracks along the waterfront.

So far, these fishers have expressed that they want responsible management. They advocate for science that supports their livelihoods. They want more research. They don’t all see eye to eye on every issue, but as far as I have heard, science is not the enemy.

This is just the beginning for me, and I am sure that I will interview people with more divergent opinions than my own. And it will be a challenge for me to steer the conversation in the right direction, but I am confident that I will be able to do it. Divergent opinions, as long as they don’t harm other people, I believe are healthy for society. I love listening to how other people see the world, with a grain of salt. And sometimes, beautiful narratives emerge.

When I was first being trained by Beth, she was telling me and my other mentor, Dr. Tommy Swearingen, about an interview she had just completed that had brought her to tears. She told me that there is something about the openness of the interview environment that allows people (both the interviewee and the interviewer) to divulge stories that in typical settings wouldn’t be discussed. On my fourth interview in Newport, I experienced a genuine, moving moment like this. I asked him if his life as a fisherman was fulfilling, and as he spoke I could tell he loved his line of work. He said every morning he got up at 4:30 am, made his black coffee, made plans for the day, and couldn’t wait to venture out on the boat. I wish you could have heard him say this, for I could feel his joy and it made my eyes blur. He loves this life.

I get paid to be moved by the stories of others. I cannot be more grateful that this is how I am spending my summer.

The Newport bridge, which I cross on my journeys. I wonder where I will go next?

NOAA Fisheries Fish Cutting Party

In an effort to escape the snail pace of cubicle work I reached out to my Marine Biology professor at Hatfield Marine Science Center to discuss possible field opportunities. I was very fortunate that professor Itchung Cheung (also how I learned about the Summer Scholars internship) recommended an upcoming NOAA event at Hatfield. So on his recommendation, last week I was fortunate to attend the NOAA Fish Cutting party 2018 during which we processed 1981 salmonids (primarily coho and chinook). The goal of the Fish Cutting party is to process the samples that are collected annually during the Juvenile Salmon Sampling Program. To process a juvenile salmon a number of samples and measurements are needed.

Transect map of fish collection sites for annual juvenile salmon sampling program

Transects sampled for coho and yearling and subyearling Chinook salmon, 1998 –present. (provided by NOAA Fisheries)

To begin, a length and weight are often collected and then the fish is scanned on a metal detector to indicate presence of a coded wire tag, pit tag, or combination of both. Coded wire tags (CWT) provide information regarding the origin of the fish or which hatchery it was released from. These metal tags are smaller than a grain of rice and usually inserted into the snout of the fish. Pit tags, about the size of a grain of rice, provide information such as migration patterns, migration timing, and growth rates by comparing size/length between when the fish was initially tagged and when it was collected. These tags are usually inserted beneath the skin on the abdomen of juveniles. Pit tags use passive technology, utilizing scanning arrays that are placed in their migration corridor within dam fish ladders and on the bottoms of streams. When a fish with a pit tag swims across the array, the information is automatically uploaded to a global database. You can literally track a fish migrating downstream and returning through the database each time the tag is scanned, assuming the fish survives the perilous journey. Coded wire tags require removal of the tag to read which is lethal to the fish and does not provide information about migration patterns, rather just hatchery origin.

A table with multiple salmon ranging from 12 inches to 20 inches known as jumbo salmon

Jumbo Chinook salmon are salmon that do not follow the normal northward migration and rather reside off the coastal waters of their native streams. (photo by Wesley Noone)

Volunteers during the Fish Cutting party had the tasks of removing fish snouts for CWT and pit tags (if present) as well as any combination of the following: removal of the stomach, otolith (ear bone), intestines, and fin. Each specimen serves a purpose in the ongoing ecological monitoring of salmonids in the Pacific Northwest. Stomachs provide valuable information regarding what salmonids are eating out at sea and where certain foods are available. Plastics have also been observed in stomach contents which is a concern especially moving into the future. Otoliths are the tree rings of fish and provide information regarding how rapidly a fish is growing. Otoliths can also have a distinctive mark that is formed during the early stages of life during hatchery rearing by controlling water temperature fluctuations. These distinctive marks can be an alternative to CWTs and provide information about hatchery origin. Intestines can be used to further analyze diet intake although determining the prey source at this stage of digestion is far more difficult. A portion of a fin is also removed primarily for genetic sampling. Other data that is collected includes any observations of parasites and visceral fat which develops on the outside of the stomachs in hatchery origin fish due to the high protein content of their diet within the hatchery.

Sea grant intern Kong Vang dissecting a small juvenile salmon

Kong Vang dissects a juvenile salmon in search of a stomach (photo by Wesley Noone)

Performing this kind of sampling on an annual basis allows ecologists to monitor the health of different populations as well as the species as a whole. This was my first experience dissecting fish for biological sampling although I have gutted many fish in my free time to take home and eat. I would recommend the next Fish Cutting party to anyone interested in helping out with the monitoring effort but I do have some reservations for those not experienced with handling fish. My first reservation is to hold off on eating lunch until you get past the nausea that is induced by the smell of hundreds of fish being processed and digging through goopy brains to find otoliths. I would also recommend that you prepare yourself for having fish tissue flung at your face as well as handling a dead animal with feces extruding from its body. Some of these fish are hosts for parasites so if that freaks you out maybe fish cutting parties are not for you.
Overall I had a great three days in Newport meeting people from the NOAA Science Center, hanging out with fellow scholars, immersing myself with fish guts, and learning new skills. Thanks to the fellow Sea Grant Scholars who showed up to help and those scholars that helped make our experience at Hatfield enjoyable.

three volunteers working on dissecting fish

A happy crew of fish cutters work on their specimens. Top left: Will Fennie (OSU Graduate student) Front left: Kong Vang (NOAA Fisheries Summer Scholar) Right: Abby Ernest-Beck (EPA Summer Scholar) (photo by Wesley Noone)

Tracing water quality from the rivers to the bay

I never thought I’d spend so much time in a river as part of a marine science research project, but here I am and I’m having a blast!

The Newport EPA has been going on research cruises to monitor water quality in the streams and the bay of Tillamook 1-2 times per month for a year now. Two weeks ago, I got to participate in the last cruise of the study. We divided into two teams, the “bay team” and the “tributary team.” Each team took measurements of dissolved oxygen, temperature, depth, salinity, and chlorophyll on-site using a multi-parameter data sonde. We also took water samples to analyze in the lab for carbonate chemistry, nutrients, dissolved organic carbon, and dissolved inorganic carbon; each measurement requires a different type of container for the sample. To make all of this easier, the EPA has converted a trailer into a mini-lab for field sampling. We have cabinets, countertops and lots of equipment all organized and easy to access!

By monitoring many different water quality parameters at locations throughout the rivers and the bay, we’re hoping to get a holistic view of the water chemistry in this system and to identify the drivers of any changes observed in the health of the system.

The trailer rigged for water sampling on the go.

Filling a cooler with a variety of water samples. Not pictured are the DOC samples, which are placed on dry ice so that they freeze immediately.

Another part of the picture is looking at how in-stream processing changes the water chemistry, specifically the amounts of carbon and oxygen. If we can get an idea of this, then we can know which changes in water quality are due to in-stream processing versus inputs such as agricultural runoff as the river runs from the forests to the bay.

Most in-stream processing is driven by periphyton, the algae growing on the rocks at the bottom of the river. To measure these changes, we removed all the rocks from a small (about 1 square foot) area in the river and placed them in a sealed container. We measured the initial dissolved oxygen in the water, let it sit in the river for four hours, and then measured the oxygen again.

Setting up the containers to measure in-stream processing. There were 4 containers with rocks and 2 controls with only streamwater at each of the two locations.

Jody Stecher, left, and me, right, measuring the oxygen in the container after removing it from the river.

We also wanted to see how one parcel of water changes as it moves downstream. At our upstream location, we released a bag of oranges into the water. Since they float in the water, they move at about the same rate as the water, so we were hoping to take measurements when they reached the downstream location. However, after about 4 hours of waiting, we decided to let this part of the experiment go. Lesson learned: there are a lot of orange leaves in the river that look like oranges when that’s what you’re hoping to see!

Waiting for the oranges to come.

After taking so many measurements and samples in a relatively short period of time, we have quite a bit of lab work and analysis coming up! Some samples will be sent to other facilities for analysis, and some will be analyzed here. I’m excited to start making sense of these data and bring together the many parts of this project to understand the system as a whole.

 

4 Million Years of Fish

This lamprey is waiting to be tagged

Often inaccurately referred to as “lamprey eels,” this slippery creature is actually a jawless fish that dates back millions of years. Richard Litts, monitoring coordinator for the Tenmile Lakes Basin Partnership (TLBP), teamed up with Statewide Lamprey Coordinator Ben Clemens and Doc Slyter, Elder of the confederated Tribes of Coos, Lower Umpqua, and Siuslaw Indians, to monitor lampreys in the area. Litts and Clemens were there as a continuation of a data collection project monitoring lamprey population trends, while Slyter provided some cultural context for the importance of lampreys. I spoke with him about the history of lampreys in the area and what they mean to the tribe, and he told me that he used to take his kids swimming in these rivers when there were “hundreds” of lampreys among the rocks. “Now,” he says, “you’re lucky to see one at all. If these ancient creatures are being this severely affected, then something’s wrong.”

Several volunteers, members of the watershed council, a veterinarian, and a couple of South Slough interns all gathered together yesterday to try to figure out what that “something” was. The process began by stationing volunteers with nets at all the possible escape points just downriver from the backpack shocker, used to stun fish into the nets. The process sounds simple enough, but lampreys are excellent escape artists and we did several rounds before we caught a fish.

When the man in the backpack yells, “Shocking!” you better have your hands out of the water and your nets in the rocks, because lampreys are swimming your way.

If and when we do catch something, it’s collected in a bucket like the one shown above. When we think we’ve hit the max for the day, the fish are put in a big tub of diluted MS222, the equivalent of anesthesia for fish. When they are motionless, they are quickly picked up, weighed, and measure by length and girth.

Putting the lamprey on the scale.

Lamprey getting its measurements taken. It needs a minimum 85mm girth to be radio tagged.

Then, a small portion of the caudal fin is taken for genotyping and they’re sent off to the “surgery station”

A volunteer handed me her special scissors and let me have a go at DNA collection!

One – by – one, these vials are filled with lamprey DNA for genotyping.

The “surgery station” was put together by members of this group. The tub is filled with diluted MS222 and PVC pipe supports the lamprey while it is being tagged. The tube has a cloth where the lamprey sit and a sponge at the bottom to keep its head from being damaged while allowing it to remain partially immersed in the anesthetic. The radio tag is activated, then surgically inserted and the lamprey is sutured up. Post-op, the lamprey are put in baskets situated in the river where they can safely wake up and eventually be released back into the current

All prepped and ready to go! The “surgeons” admitted that all the bananas in their house have been tightly sutured as their practice runs.

After all seven had been tagged, the group sat down for lunch together and discussed everything they learned about lamprey and lamprey monitoring that morning. One of the interns expressed how rewarding it is to be around so many people with a common goal, and I found a similar contentment in knowing that everyone here feels strongly enough about the protection of these ancient fish to spend their morning in the river.

Endangered Species Act

My work this summer focuses on the Endangered Species Act (ESA). The ESA is a law that was implemented in 1973 which recognizes “species of fish, wildlife, and plants are as esthetic, ecological, educational, historical, recreational, and scientific value to the Nation and its people.” Under this law it is prohibited to take an endangered or threatened species also known as listed species. The purpose of ESA is to protect and conserve listed species and their ecosystem, so that the species can recover and self-sustain itself without further protection by federal agencies in the future.

ESA is the backbone of NOAA Fisheries and the entirety of work they do. This is because NOAA Fisheries allows authorization of take whether it be direct or incidental of listed species under their jurisdiction. This allows states, privates, federals, and tribes to proceed with their programs knowing they will not violate the ESA only if the program does not jeopardize or imposes any adverse modification on the critical habitat.

Learning and understanding the ESA was a big challenge. The next step of my project is to determine and distinguish the different pathways of ESA. Whether the programs or projects proposed by state, federal, or tribe falls under one of the 4(d) limits, section 7 or section 10, all of which allows some form of take or incidental take. If you aren’t lost already and have no clue what I’m talking about. It is totally fine, because my goal by the end of the summer is to make the processes digestible for the applicants.

The work I do does not involve much field, however I did get the opportunity to visit some habitat sites a co-worker of mine has worked on involving section 7 consultation. In addition, Wes the other OSG summer scholar and I had the opportunity to attend a meeting up in Washington. On our way back home, we took a detour to a NOAA retiree’s house, where we harvested clams and oysters for the first time. Taking about clams, I should cook some now. Until next blog, I’ll let you know about the boat trip and the salmon hatchery tour.