The Hidden Side of Graduate School: Finding your place within your discipline

Summary: Graduate student researchers Brian Erickson and Chelsea Behymer talk about their transition from natural sciences to social sciences and the process of finding their place within their disciplines.

As graduate students, many of our academic conversations focus around our research. But graduate school is about more than just designing and carrying out a project; it also involves finding your place within a larger community.

Chelsea Behymer and Brian Erickson met through a science communication course in the Integrative Biology department (IB599), and they quickly found common ground. Although their research interests are very different, both have had experiences that sparked interest and conviction to explore the human dynamic of the ecological systems with which they are more familiar. While neither is new to academia, they find themselves navigating new identities as they explore what it means to be a social scientist working on human components of environmental issues.

Chelsea takes guests onboard a coastal Alaskan expedition on an intertidal walk.

Chelsea is a first-year Ph.D student in the Environmental Sciences graduate program, with a focus on informal science education. For the past six years, Chelsea has engaged diverse groups of people in marine biology and natural history as a Naturalist onboard both large and smaller, expedition style cruise ships. Interacting with a diversity of people in shared travel and learning experiences across the world’s oceans has been one of the most rewarding roles of her career. At the same time, being immersed in nature-based tourism has opened her eyes to the nature-based tourism industry as not only a place where human connections to the natural world are fostered, but provides wonderful opportunities for science communication.

With the growing nature-based tourism industry, perhaps the opportunities to connect have never been more abundant. Chelsea’s research interests aim to understand the potential for citizen science in nature-based tourism to act as both an effective means of engaging people with local scientists, while at the same time providing opportunities for the kind of collaborative environment where meaningful conversations between scientists and the public can occur.

Brian presents work on ocean acidification education during the State of the Coast conference.

Brian is also a first-year Ph.D student studying fisheries social science in the Department of Fisheries and Wildlife. Growing up in the midwest, he first fell in love with the ocean while working as a field technician in the US Virgin Islands, Panama, and the Northwest Hawaiian islands. Partially because he defined himself as a biologist, it took him almost a decade to realize that he was interested in answering social science questions. Brian is generally interested in applying what we know about human behavior to improve marine conservation outcomes for people and the planet. His master’s work at OSU focused on exploring a commonly held assumption – that knowledge of environmental problems leads to action to fix those problems – through the lens of a high school ocean acidification curriculum. For his PhD work, Brian will be collaborating with the SMART Seas Africa Programme to examine social aspects of marine conservation in East Africa.

In this special segment, Chelsea and Brian will talk with ID host Kristen Finch about the challenge of finding their way as social scientists in a field that is working towards interdisciplinary collaboration. Don’t miss this conversation; tune in to KBVR Corvallis 88.7FM at 6 pm PST on Sunday March, 10. Stream the show live or catch the podcast.

Written by Chelsea Behymer and Brian Erickson. Edited by Kristen Finch.

Who Runs the World? Exploring Gender Diversity in the Forest Sector

The following article was written by Pipiet Larasatie and edited by Kristen Finch.

Pipiet Larasatie is a third year PhD student in Wood Science and Engineering Department, College of Forestry, at Oregon State University. Her friends and close colleagues describe her as “Ms. Social” and “Ms. Doing-All.”

And she is! Pipiet is currently involved with four research projects and has standing on four committees at the Department and College level (e.g. College of Forestry’s Diversity Equity Inclusion Committee). Additionally, she is a digital communications coordinator for the International Society of Wood Science and Technology. One of her initiatives is #WomenInWoodScience or a network for women who are associated with wood science.

Pipiet working in the Forest Sciences Dept. University of Helsinki in 2017.

As a woman and a first generation student in her male dominated family, Pipiet has a high passion on empowering young females. For this reason, Pipiet switched her research focus from wood centric to gender diversity in the forest sector.

So far, Pipiet’s research involved collaboration with folks at OSU (her advisor and a Master’s student), but also international collaboration with a professor and a Master’s student in University of Helsinki, Finland. During this part of the project, the team interviewed female executives in the global forest sector companies about gender aspects in the North American and Nordic industries. Some trends became apparent across interview responses. Their respondents agreed that the North American and Nordic forest sector is a historically male-oriented and male-dominated industry, which can lend itself to characteristics of a chauvinistic and masculine culture. This also was clear: to be successful in the male-dominated work setting, young females need a support on multiple levels e.g. good bosses/leaders, mentors, and networks. The interviewees also voiced that education is important when finding a niche in the workplace and for making young females more competitive in the job market. 

Pipiet with one of her mentees joining a faculty led summer course, “The Forest Sector in Alpine Europe.” Photo shows group at University of Primorska, Slovenia.

Tune in to KBVR Corvallis 88.7FM to hear our special segment with Pipiet at 7 pm on March 3, 2019. Pipiet present her research findings alongside pop songs from Beyoncé and Alicia Keys. Later, Pipiet will be accompanied by one of her mentees, Taylor Barnett, a third year undergraduate student studying Natural Resources at College of Forestry. Taylor will share her experience with mentorship programs at OSU and how these mentorship has aided her professional development.

Not a local listener? No sweat! Stream the show live or check out the podcast version of this special episode.

3D Modeling Rock Shape: Archeological Research of the Earliest North Americans

At age 17, like a lot of teenagers, Samuel Burns wanted to go to college. Unlike most college-bound 17-year-olds however, Samuel didn’t have a high school degree. Today, Samuel is a first-year master’s student in Applied Anthropology, within the School of Language, Culture, and Society, and the Department of Anthropology. Also, this is his second master’s degree.

Samuel in the field in the Allegheny National Forest, Pennsylvania. Photo by Samuel Burns.

Samuel works with Dr. Loren Davis to investigate the earliest archeological sites in North America, and there are two big questions to answer: when did humans first arrive in North America, and by what route did the earliest humans arrive? Traditionally, humans are thought to have entered North America through the Rocky Mountains, but more recent evidence suggests that maritime cultures may have arrived first, finding North America via the ocean. The oldest fish hooks in North America are somewhere between ~11,300 to 10,700 years years old and were discovered off the coast of Baja California, Mexico on Cedros Island.

Cedros Island is just one of two archeological sites of interest for Samuel’s research group, and while he has been to Cedros to conduct fieldwork, Samuel’s work focuses on artifacts from one pit in the second site: Cooper’s Ferry in Cottonwood, Idaho, near the Salmon River. From Cooper’s Ferry, seemingly interesting artifacts are brought back to the lab where they are sorted, confirmed to be artifacts, and studied.

L-R: Loren White (OSU), Steve Jenevein (Oregon State Parks), and Samuel Burns on board the flight from Cedros Island, Baja California, Mexico after a successful field session in January, 2019. Photo by Samuel Burns.

Samuel is able to take the artifacts, make 3D scans of the object, and input this information into a computational program. The computer converts the 3D scans into mathematical shapes and 3D models. So instead of looking at a couple things by eye and estimating if artifacts are similar or different, the program can compare large sets of data with discreet numbers and make conclusions about whether or not two artifacts found in different places have similar shapes. This allows researchers to ask questions about tool development over time and place.

To make 3D images, a laser scanner has been used in the past, but this is both expensive and large, so new methods are actively being developed for this purpose. One option is a structured light scanner, which has a light shining through multiple holes. To use a structured light scanner, you place your artifact on a patterned background and take lots of photos at many angles, producing a large amount of data to feed the computer program. Another easier option for 3D modeling is photogrammetry, which only requires a camera and a computer, even just a phone camera will work. This soft ware used is called “GLiMR” (GIS-based Lithic Morphometric Research) and is based on GIS software for modeling geographical landscapes, and the automation and ease of such a program enables archeologists to spend less time collecting numbers and more time assessing these numbers through statistical analyses and asking interesting questions.

Samuel’s crew lining up to conduct a systematic surface survey near Paulina, Oregon. Photo by Samuel Burns.

When you think about ancient North American stone artifacts, megafauna hunting tools like arrow heads and spears come to mind. However, in both the Cedros and Cooper’s Ferry sites, simpler tools are being found that suggest early North Americans exploited a wide range of resources and had a broad-spectrum diet. For example, artifacts found include shell or stone tools for processing fiber to making fishing line.

Samuel using a digital total station to take measurements at a Medieval Christian period site at el Kurru, Northern State, Sudan. Photo by Walter De Winter.

Growing up, Samuel never went to school and wasn’t homeschooled, but always loved history. He lived in an 1850s farmhouse, and spent his childhood going through old objects from his backyard, left behind over the past 100+ years. At age 17, realizing he wanted to go to college but not having the traditional requirements, Samuel applied to a University in Jerusalem and got in. After spending a year there, he ran out of money, and spent next few years working and moving around the world, including in South Korea and Israel. Eventually, he returned to the US and jumped back into school at a community college in Michigan and ultimately transferred to the University of Michigan, where he focused on ancient cultures and language of middle east.

Field camp near Colt, Arkansas, home for 6 months in 2016-2017. Photo by Samuel Burns.

Samuel graduated from UM in 2010 and then got a master’s degree at the University of Cambridge in the United Kingdom, focusing on Egyptian studies. This first master’s centered around Syria and unfortunately, this research project was not able to be pursued further, so Samuel spent the next five years working in cultural resource management in the US. Through this job, he was able to travel around the US and soon became interested in North American archeological research. Samuel had a strong liberal arts background but, wanting to expand his earth science knowledge, came to Oregon State.

Eventually, Samuel wants to obtain a PhD and work in academia, continuing to formulate and direct research projects.

To hear more about Samuel’s path to OSU and experiences in archeological research, tune in Sunday, February 16th at 7 PM on KBVR 88.7 FM, live stream the show at http://www.orangemedianetwork.com/kbvr_fm/, or download our
podcast on iTunes!

 

Davis, L. G., Bean, D. W., Nyers, A. J., & Brauner, D. R. (2015). GLiMR: A GIS-Based Method for the Geometric Morphometric Analysis of Artifacts. Lithic Technology, 40(3), 199–217.
Des Lauriers, M. R., Davis, L. G., Turnbull, J., Southon, J. R., & Taylor, R. E. (2017). The Earliest Shell Fishhooks from the Americas Reveal Fishing Technology of Pleistocene Maritime Foragers. American Antiquity, 82(3), 498–516.

Feather collections and stressed-out owls

Ashlee Mikkelsen holding a juvenile northern spotted owl. Photo courtesy Ashlee Mikkelsen.

For six months out of every year, Ashlee Mikkelsen spends her days hiking for miles off-trail in the Ponderosa pine-filled forests of central Washington, hooting like an owl, and carefully listening for responses. These days, responses can be few and far between. You see, Ashlee isn’t just a wildlife enthusiast; she is a research assistant in a long-term US Forest Service monitoring program focused on the northern spotted owl.

Since being listed as threatened by the US Fish and Wildlife Service in 1990, populations of northern spotted owls have continued to decline. In some areas, the number of spotted owls has decreased by more than half in only 20 years (see (Dugger et al. (2016)). Northern spotted owls are inhabitants of old-growth forests. Although northern spotted owls historically could be found in almost every forest from northern California to British Columbia, as forests have shrunk in size through timber harvesting and through changing land use, the amount of suitable habitat has drastically decreased. A second major contributor to the decline of the northern spotted owl is arrival during the last century of the barred owl, which are native to northeastern North America. Barred owls competed with spotted owls for territory and resources, and have been observed fighting with spotted owls.  Ashlee’s master’s research at Oregon State aims to quantify the stress experienced by spotted owls.

Northern spotted owl. Photo courtesy Ashlee Mikkelsen.

When birds experience stress, their bodies respond by releasing larger-than-usual quantities of the hormone corticosterone. Similar to cortisol in humans, corticosterone is always present, but having levels that are very high or that are very low is associated with poor health outcomes. It used to be that in order to measure the physical stress response of a bird, researchers had to take a blood sample. The problem with this is that the process of taking a blood sample itself is a source of stress for the bird. Recently, however, a new technique was introduced based on the fact that corticosterone is also present in feathers. Being able to use feathers is a distinct advantage: birds are constantly dropping feathers, so collecting feathers is fairly non-invasive, and importantly, similar to the benefits of measuring cortisol in hair, feather corticosterone measurements show the average level of the hormone over a long period, rather than just the instant that the feather is collected.

Ashlee banding a juvenile northern spotted owl. Photo courtesy Ashlee Mikkelsen

Ashlee banding a juvenile northern spotted owl. Photo courtesy Ashlee Mikkelsen

Working with professor Katie Dugger (who, incidentally, was Ashlee’s supervisor in the owl-monitoring field crew for the two years prior to beginning graduate school), Ashlee is analyzing a collection of feathers that spans over a 30-year time period. Measuring corticosterone levels in feathers is a high-tech process involving organic chemistry and radioactive isotopes. Although there are many complications that need to be accounted for, tracking the levels of corticosterone in these feathers gives Ashlee insight into the impact of stressors such as environmental degradation and competition with barred owls. Because the data spans so many years, she is able to examine the average stress in spotted owls over periods of change in the populations of barred owls. Ashlee’s data shows a strong response in corticosterone in spotted owls when the number of barred owls in the neighborhood goes up. This supports the view that spotted owls’ woes are not just due to habitat loss, but also due to competition with barred owls.

To hear more about Ashlee’s path to OSU, experiences in research, and of course about northern spotted owls, tune in Sunday, February 16th at 7 PM on KBVR 88.7 FM, live stream the show at http://www.orangemedianetwork.com/kbvr_fm/, or download our
podcast on iTunes!

 

Saving the blue whales of the South Taranaki Bight

A blue whale engulfs a patch of krill. Drone piloted by Todd Chandler.

Until a worldwide ban took effect in 1986, whaling and the production whale products, were leading to a decline in whale populations. Despite a greater global awareness about the importance of protecting our oceans, conflicts still exist between conservation efforts and industry.

This week’s guest, Dawn Barlow, studies the anthropogenic effects on blue whales (Balaenoptera musculus) – the largest known animal to have ever existed! Dawn is a first year PhD student in the Department of Fisheries and Wildlife’s Geospatial Ecology of Marine Megafauna (GEMM) Labwith Dr. Leigh Torres – the same lab where she completed her Master’s degree in 2018.

A blue whale mother and calf surface near Cape Farewell, New Zealand. Photo by Dawn Barlow.

Discovery of new whale population… and problem

Through her Master’s work, Dawn and her colleagues were able to document a genetically distinct population of about 700 blue whalesin the South Taranaki Bight (STB) – a region located between the north and south islands of New Zealand. The STB is not only an important region for the blue whales; however, it is also heavily used by industry, with active oil and gas extraction, seismic surveying, shipping traffic, and proposed seafloor mining. The need for a marine sanctuary in this area is eminent for the longevity of this whale population, but a compromise must be reached with the government and stakeholders. Furthermore, defining a sanctuary area in a dynamic system is not as simple as drawing a line in the sand.

Data collection Down Under

A pair of blue whales surface in New Zealand’s South Taranaki Bight region. Photo by Leigh Torres.

For her PhD research, Dawn will be continuing work with this same population of whales to get a better understanding of the ecological factors that influence where the blue whales are distributed. So far, three data collection trips have been conducted to gather some of this information. These ship-based trips have collected huge amounts of data using a myriad of equipment and techniques.

Echosounder data is collected using a transducer, which hangs off the boat and sends two pings per second producing measurements from the bounce back that can be used to map out krill aggregations – the blue whale’s primary food source. Conductivity, Temperature, Depth (CTD) casts are used to collect temperature and salinity pressure measurements to determine depth. Wind measurements are also recorded, as this generates upwelling. Photography and videography from the ship deck and via drones are used for identification of individuals whales with their skin providing the equivalent uniqueness as a human fingerprint. Satellite imaging is also used to record sea temperatures and chlorophyll levels. Lastly – and my personal favorite – darts shot from a smaller inflatable boat at close-range are used to collect skin and blubber samples for downstream genetic, stable isotope, and hormone analysis. Opportunistic sampling of fecal matter (i.e. if a whale poops) can also be used for genetic and hormone analysis.

Approaching a blue whale for photo-identification and biopsy sampling. Photo by Kristin Hodge.

Dawn participated in the 2017 field season and also went in July 2018 to disseminate findings to stakeholders. Now she is tasked with sifting through the data to correlate the oceanography with acoustic data, satellite imagery and presence of krill. Preliminary results suggest that the blue whales seem to appear where krill aggregate. Through habitat modeling on an ecosystem scale, Dawn hopes to be able to predict on a seasonal scale where the krill – and therefore, blue whales – will be, allowing for informed, science-based conservation and management decisions to be made.

Finding a passion for conservation biology

Dawn Barlow on the flying bridge of the research vessel during fieldwork in New Zealand. Photo by Kristin Hodge.

Growing up in Northern California near the ocean has always inspired Dawn to pursue a career in marine science. Dawn received dual bachelor’s degrees in Organismal Biology and Environmental Policy at Pitzer College in Claremont, California, where she recognized the need to build a bridge between biology and its translation to conservation policy. Knowing she wanted to get hands-on experience in marine mammal research, Dawn sought out and pursued opportunities through the MARMAM listserv, which landed her two undergraduate internships: one studying bottlenose dolphins in Australia and another in Alaska with humpback whales. These internships allowed Dawn to realize her desire to continue research through a graduate program at Oregon State University, where she has already completed her Master’s degree in Wildlife Science. After completing her PhD, Dawn plans to continue conducting conservation research.

Join us on Sunday, February 10 at 7 PM on KBVR Corvallis 88.7 FM or stream live to learn more about Dawn’s adventures Down Under, journey to graduate school, and answer to the age-old question: what does whale poop look like?

Exploring immigrant identity through poetry

As a 2nd year MFA student in the School of Writing, Literature, and Film, Tatiana Dolgushina is writing her history through poetry as a way to understand herself and the country she came from that no longer exists. Born in Soviet Russia, Tatiana and her family fled the country after it collapsed in 1991. Tatiana grew up in South America and came to the US when she was 12, settling in Ohio. She remarks, “so much cultural history of Soviet Russia is influencing who I am today.” Central to her work are ideas of identity formation and childhood displacement. Through writing, she is digging deeper into her experience as an immigrant growing up in multiple countries.

To better understand the root of her identity, Tatiana is reading about the history that led to the dissolution of Soviet Russia. Reading about the history has helped her to understand the events that led to her family’s displacement. She grew up with silence surrounding why they had left, explaining, “Soviet culture is based on a fear of talking about historical events.” She reflects on feeling shame associated with being an immigrant, and in “not belonging to the old place or the new place.” A fractured in-between place. “As a kid, when you’re displaced, you lose so much: language, traditions, and culture.” She further explains, “you seek assimilation as a kid, and either forget these things, or push them away.”

Tatiana explains that poetry is a catalyst for understanding herself and more broadly, for us to understand ourselves as humans. It’s about connecting the dots. Her family doesn’t speak about what transpired. But reading the history, it begins to make sense. “When you’re a kid, you’re focused on survival.” She reflects that she has been trying to compensate for certain things, and is now understanding how and why she is different. She realized, “the older I get, the more I feel it, my immigrant self emerging.” Her experience growing up in multiple countries has contributed to her identity formation, but she admits that she doesn’t have a space to talk about it. “I blend in, but still feel like an outsider. I am not of this culture, and I realize that I really have no home because my home is not a country.”

Tatiana is still trying to figure out what her writing is about, but articulates that writing is a process of not being able to say certain things in the beginning. It’s about writing through the memory and being able to see the things you need to see when you’re ready, peeling away each layer of experience. Approaching the writing process linearly, Tatiana began writing about early memories, then proceeded beyond to older memories, asking, for example, “why did I write about that nightmare I had when I was 4 years old?”

Originally trained as a wildlife biologist, Tatiana decided to change directions after spending time pursuing a Master’s degree. When she initially began the MFA program, she was shocked at the discussion of subjective ideas, which is so different from many areas of scientific discourse. In science, the focus is not so much on identity. But, she explains, “science and art are coming from the same place. It’s about observation, and understanding through observation.”

As a personal goal, Tatiana is working towards publishing a book. It has been something she has wanted to do for many years. “The hope is that a 15 year old immigrant kid in the library will read it and be able to relate to my story.”

Tatiana studies with Dr. Karen Holmberg and will be graduating this Spring. Tune in on Sunday, February 3rd at 7pm on KBVR 88.7 FM to hear more from Tatiana about her thesis work and experience as a graduate student at OSU. You can also stream the show or download our podcast on iTunes!

Sticks and stones may break my bones, and words might unintentionally enforce gendered behavior

Hey guys, do you notice when you or others use gendered language? As with the last sentence, gendered language has become part of our culture’s vocabulary and we may use it without a second thought. There is a growing field of research that studies how language can shape perceptions of ourselves and others.

Jeana presenting “Decolonizing Masculinities” with Nyk Steger and Minerva Zayas at the 2018 Examining Masculinities Conference at OSU

Jeana Moody is a second year Masters student in Women, Gender and Sexuality Studies working with Professor Bradley Boovy. Her thesis research focuses on the use and impact of gendered words and phrases in the English language, such as “throw like a girl”, “man up”, and “don’t be a bitch.” What are the implications of saying “man up” to someone who presents as a woman? As a man? Does the gender of the speaker play a role?

To explore this, Jeana designed a study to collect data through in-person interviews and anonymous online surveys, asking participants to describe situations when they have either used such statements or have been the subject of the statements. The questions include: where did this happen? Who was there? Were there any power dynamics? How did it make you feel then, and now?

For any research involving human participants, OSU researchers must submit a proposal to and be approved by Oregon State’s Institutional Review Board (IRB). This rigorous process requires submission of interview questions, the number of participants, how the data will be collected, and how consent will be obtained from the participants. Additionally, since there is always the possibility of triggering a participant’s traumatic memories from survey questions, help resources must be provided to participants. Jeana’s study was just approved last week.

Jeana hiking in the San Gabriel Mountains in Southern California

From the data collected, Jeana hopes to gain insight into feelings of and implications on participants in the study, and present the anecdotal evidence within a cultural context. This research draws from the subjects of feminist sociolinguistics and critical race theory. It addresses the idea that language begets culture, and culture begets language. Her interest in the subject arose from working with non-native English speakers. She observed that they often use American swear words and racist words without understanding the impact of the words they were using. Just because someone doesn’t understand those words doesn’t mean they don’t hold an impact.

Jeana hiking in the Willamette National Forest in Oregon

When Jeana is not conducting research, she is the instructor of record for Men and Masculinities and is a Teaching Assistant for several other classes. She is originally from Pullman, Washington and attended Western Washington University as part of the Fairhaven College (an interdisciplinary liberal arts college). She enjoys hiking and being anywhere outdoors, and she loves to cook and draw. When not in Corvallis, she can likely be found in Prague where she has taught English and worked for a travel agency.

If you are interested in participating in Jeana’s research study online or in-person, please email moodyje@oregonstate.edu to set up an interview or with any questions you may have, or follow the link to her Gendered Language Online Survey.

Written by Maggie Exton.

Kayaks and Computers: the Gray Whale Research Essentials

Throughout the year, looking out from the Oregon coast, you can often spot gray whales with the naked eye. Behind the magic and mystery of these massive creatures are teams of researchers tracking their migration and studying their diet.

Lisa Hildebrand is a 1st year Master’s student in Wildlife Science working with Dr. Leigh Torres within the College of Agriculture. Lisa studies geospatial ecology of marine megafauna, meaning that her research focuses on the feeding and movement through time and space of sea creatures larger than most fish, including large sea birds, seals, dolphins, and of course, the gray whales. To study such large animals in the ocean, Lisa manages a team that combines diverse technologies coupled with fine scale foraging ecology.

Gray whales feed on very small zooplankton suspended in shallow water. The whales don’t have teeth but instead have rows of baleen which look like a thick brush and act as a filter for water and sediment while letting in large quantities of zooplankton. In July and August, Lisa and her team of 4-5 people go out to Port Orford, Oregon. The team splits into two groups: a cliff team and a kayak team. From a cliff above their 1km2 sampling site, theodolites and computational programs are used to track whales by height and GPS location. Once a whale is spotted, team members kayak to this location and take water samples for analysis of zooplankton density, caloric content, species, and microplastic quantity. Lisa has taken over this ongoing project from a previous Master’s student, Florence Sullivan, and has data on the same research site and whales going back to 2015.

This research project provides opportunities for both undergraduates and high school level students to obtain first-hand field research experience. The students involved are able to take what they’ve heard in a classroom and apply it outdoors. In particular, Lisa is passionate about getting the students in the local Oregon coastal community involved in research on the whales that bring many tourists to their area.

To study the large gray whales, Lisa spends most of her time studying the small zooplankton that they eat. Zooplankton hide under kelp and it turns out, can be separated by populations that are pregnant, or varied in age or species. Gray whales may show preference for some feeding sites and/or types of zooplankton. Why do we care what a gray whale’s dietary preferences are? Plastic use and plastic pollution are rampant. Much of our plastic ends up in the oceans and photodegrade into microplastics small enough to be consumed by zooplankton. Since gray whales are the top predator for zooplankton and eat large qualities, these microplastics accumulate. Microplastic presence may differ between regions and species of zooplankton, which may relate back to whale preferences and migratory patterns. On the Oregon coastline, microplastic profiles of zooplankton have not yet been studied. As humans are also consuming large quantities of seafood, it is important to understand how microplastics are accumulating in these areas.

Lisa is from Germany and grew up in Vietnam and Singapore, but she was first inspired to pursue marine animal research as a career after a family trip to Svalbard, Norway during high school. Before obtaining her undergraduate degree in Marine Zoology from Newcastle University in England, Lisa took two years off from schooling and completed two internships: one with bottlenose dolphin sanctuary research institute in Italy and Spain, and one at a seal research facility in Germany. Now that she’s settled in Oregon for now, Lisa is enjoying the nature and in her free time loves hiking and skiing.

To learn more you can check out GEMM Lab website , the GEMM Lab blog and Lisa’s Twitter, @lisahildy95

To hear more about Lisa’s research, tune in Sunday, January 20th at 7 PM on KBVR 88.7 FM, live stream the show at http://www.orangemedianetwork.com/kbvr_fm/, or download our podcast on iTunes!

Treating the Cancer Treatment: an Investigation into a Chemotherapy drug’s Toxic Product

One of the most difficult hurdles in cancer treatment development is designing a drug that can distinguish between a person’s healthy cells and cancer cells. Cancerous cells take advantage of the body’s already present machinery and biochemical processes, so when we target these processes to kill cancer cells, normal, healthy cells are also destroyed directly or through downstream effects of the drug. The trick to cancer treatment then is to design a drug that kills cancer cells faster than it harms healthy cells. To this end, efforts are being made to understand the finer details that differentiate the anti-cancer effects of a drug from its harmful effects on the individual. This is where the research of Dan Breysse comes in.

Dan a third-year master’s student working with Dr. Gary Merrill in the department of Biochemistry and Biophysics. Dan’s research focuses on a common chemotherapy drug, doxorubicin. Doxorubicin has been researched and prescribed for about 40 years and has been used as a template over the years for many other new drug derivatives. This ubiquitous drug can treat many types of cancer but the amount that can be administered is limited by its toxic effect on the individual. Nicknamed “the red death,” doxorubicin is digested and ultimately converted to doxorubicinol, which in high doses can cause severe and fatal heart problems. However, hope lies in the knowledge that doxorubicinol generation is not related to the drug’s ability to kill cancer cells. These mechanisms appear to be separate, meaning that there is potential to prevent the heart problems, while keeping the anti-cancer process active.

Cancer cells replicate and build more cellular machinery at a much faster rate than the majority of healthy cells. Doxorubicin is more toxic to fast-replicating cancer cells because its mechanism involves attacking the cells at the DNA level. Dividing cells need to copy DNA, so this aspect of doxorubicin harms dividing cells faster than non-dividing cells. It is common for chemotherapy drugs to target processes more detrimental to rapidly dividing cells which is why hair loss is often associated with cancer treatment.

Separately, doxorubicin’s heart toxicity appears to be regulated at the protein level rather than at the DNA level. Doxorubicin is converted into doxorubicinol by an unknown enzyme or group of enzymes. Enzymes are specialized proteins in the cell that help speed up reactions, and if this enzyme is blocked, the reaction won’t occur. For example, an enzyme called “lactase” is used to break down the sugar lactose, found in milk. Lactose intolerance originates from a deficiency in the lactase enzyme. During his time at OSU, Dan has been working to find the enzyme or enzymes turning doxorubicin into doxorubicinol and to understand this chemical reaction more clearly. Past research has identified several potential enzymes, one of which being Carbonyl reductase 1 (CBR1).

Doxorubicin is converted to doxorubicinol with the addition of a single hydrogen atom.

While at OSU, Dan has ruled out other potential enzymes but has shown that when CBR1 is removed, generation of doxorubicinol is decreased but not completely eliminated, suggesting that it is one of several enzymes involved. In the lab, Dan extracts CBR1 from mouse livers, and measures its ability to produce doxorubicinol by measuring the amount of energy source consumed to carry out the process. To extract and study CBR1, Dan uses a process called “immunoclearing,” which takes advantage of the mammal’s natural immune system. Rabbits are essentially vaccinated with the enzyme of interest, in this case, with CBR1. The rabbit’s immune system recognizes that something foreign has been injected and the system creates CBR1-specific antibodies which can recognize and bind to CBR1. These antibodies are collected from the rabbits and are then used by Dan and other researchers to bind to and purify CBR1 from several fragments of mouse livers.

Prior to his time at OSU, Dan obtained a B.S. in Physics with a concentration in Biophysics from James Madison University where he also played the French horn. Realizing he loved to learn about the biological sector of science but not wanting to completely abandon physics, Dan applied to master’s programs specific to biophysics. Ultimately, Dan hopes to go to medical school. During his time at OSU, he has balanced studying for the MCAT, teaching responsibilities, course loads, research, applying to medical schools, and still finds time to play music and occasionally sing a karaoke song or two.

To hear more about Dan’s research, tune in Sunday, December 16th at 7 PM on KBVR 88.7 FM, live stream the show at http://www.orangemedianetwork.com/kbvr_fm/, or download our podcast on iTunes!

Applying medical anthropology: a history of stress in Puerto Rico and its impacts on birth outcomes

Over the course of the last six years, Holly Horan, a doctoral candidate in the Applied Anthropology program at Oregon State University, has developed and carried out a course of research culminating in the largest-ever study measuring perceived and biological maternal stress during and after pregnancy in Puerto Rico. By combining in-depth interviews with Puerto Rican mothers with quantitative analysis of perceived stress and the stress hormone cortisol during each stage of pregnancy, Holly has gained insights into both the perceived and the physiological components of maternal stress that have potential to impact birth outcomes (in particular, timing of birth).

Holly describes herself as an applied medical anthropologist. She strives to take a holistic approach to health, considering not only the physiology of an individual, but external factors as well: the political situation, economics, the culture, and the historical context of the research site. She is passionate about “community-led research.” In community-led research, the community where the research is being conducted takes a role in the development, execution, analysis, and evaluation of the research.

Holly has found a way to combine her personal and professional interests in maternal and infant health with her desire to engage in research with Puerto Rican communities. Holly’s mother is Puerto Rican, and she had long wanted to engage in research that could benefit the island. While completing a master’s degree in anthropology at the University of Montana, Holly did preliminary research on the early onset of puberty among Puerto Rican girls. Here at OSU, Holly has been able to use both qualitative and quantitative methods to research maternal and infant health within a community-led framework.

At the beginning of her dissertation research, Holly learned that the cesarean birth rate in Puerto Rico was close to 50% — far higher than the rate in the continental U.S., which hovers around 30%. Both rates are much higher than the rate recommended by the World Health Organization, which indicates that the cesarean birth rate should be no higher than 15%. She also learned that the island struggled with high incidence of preterm birth and low birth weight, both of which are important population-level health indicators. Holly’s advisor, Dr. Melissa Cheyney, is a home-birth midwife and an associate professor within the Applied Anthropology program in the School of Language, Culture, and Society. Dr. Cheyney helped connect Holly to Puerto Rican midwives, who, in turn, connected them to other medical providers in Puerto Rico.

In the summer of 2014, Holly conducted a pilot study, spending six weeks in Puerto Rico interviewing maternal and infant health-care professionals. These interviews allowed her to develop goals for her dissertation research that aligned with the needs of the community. Participant narratives frequently displayed concerns associated with unexplainable high rates of preterm birth.

Holly’s National Science Foundation (NSF)-funded dissertation research examined the relationship between perceived maternal stress, biological maternal stress, and prematurity. After the 2014 pilot study, she moved to Puerto Rico for 16 months, where she used semi-structured interviews and perceived stress questionnaires to develop an understanding of this relationship. In addition to this qualitative component, she also measured the stress hormone cortisol from maternal hair samples. Cortisol is one of the most well-understood biological stress indicators. Up until recently, the primary available way to measure cortisol levels was through blood or saliva samples, which provided only an indication of short-term stress. As it turns out, however, cortisol is also incorporated into hair. Hair cortisol provides a measure of long-term stress — the type of stress that is speculated to impact maternal and infant health outcomes, including preterm birth.

In the summer of 2016, Holly initiated her dissertation research with an extensive series of in-depth interviews with pregnant and recently-postpartum women. At this time, the ZIKA virus was declared a public health emergency, and there was a variety of public health messaging concerning delayed reproduction and the risk of microcephaly. Through these interviews, Holly learned that the U.S. Government’s public health messaging led to an internal conflict for many pregnant Puerto Rican women. Families felt stress and fear about the prospect of infants developing microcephaly. However, the warnings and official recommendations to delay reproduction provided uncomfortable reminders of the island’s colonial past, which includes targeted experimental clinical trials of oral contraceptives and sterilization offered primarily to low-income women. This led many interviewees to be skeptical about the threat of the Zika virus, but did not deter them from being concerned for their fetus’ well-being.

These participants identified sources of stress that varied widely, ranging from socioeconomic concerns, political changes, and gender-based inequalities. For example, in May 2016, Puerto Rico’s government defaulted on over 70 billion dollars of debt. Under the regulations passed by La Junta, the appointed fiscal board, many employees were fired and then rehired for lower pay. Also affected was the secondary public-school system: nearly 150 schools were closed. While these events are structural, the interviews revealed that within the Puerto Rican people, the impact of the events was personal, and the magnitude of impacts depended on individuals social support networks and life circumstances.

After comparing maternal cortisol levels with the perceived maternal stress from the structured surveys, which were collected in each trimester across pregnancy, Holly found a counter-intuitive result: some of the mothers who had most problems with their pregnancies (such as premature birth) had unusually low levels of cortisol. One current theory is the concepts of allostasis or allostatic load and “weathering,” a term which has been in the media in recently describing the cumulative effects of chronic stress on health (discussed in an NPR interview here in the context of race-based discrimination). Normally, the body responds to stress by heightening the amount of hormones such as cortisol. After the stressor is removed, hormone levels shift back to a low-stress state. However, if stress is prolonged over months or years–such as when living under a system of oppression–the body starts to experience “wear-and-tear,” causing the body’s stress response system to become ineffective. This ultimately impacts health outcomes, such as premature birth.

There have been road bumps along the way. In late summer 2017, Holly was nearly three quarters completed with data collection and the project was moving along smoothly. However, Mother Nature had different plans: In September 2017, Puerto Rico was hit first by Hurricane Irma and then by Category 4 Hurricane Maria two weeks later. The hurricanes destroyed the power grid and most of the island’s infrastructure. Holly was evacuated by OSU a week after the storm. Although she was worried about the well-being of her participants, and the impact this storm would have on the research project, NSF and her other funders graciously supported her to return and complete the study, which she did in February and March of 2018. As a separate side-project, Holly plans to return to Puerto Rico this summer to share study results with the community and with community partners.

To hear more about Holly’s research, tune in Sunday, December 9th at 7 PM on KBVR 88.7 FM, live stream the show at http://www.orangemedianetwork.com/kbvr_fm/, or download our
podcast on iTunes!