Correct answers in bold.
1. (5 points) Which of the following is NOT a property associated with DNA?
a. double helix
b. sugar-phosphate backbone
c. parallel strands (5’ to 3’)
d. nucleic acids
2. (5 points) The process by which a polypeptide (or, protein) is produced from a template RNA molecule is referred to as:
a. replication
b. translation
c. transcription
d. reverse transcription
3. You have been given a tube containing a DNA sample. The DNA is linear and 1,000 base pairs (bp) in length. To further study this DNA, you decide to use the restriction enzyme PvuII and agarose gel electrophoresis. The diagram in the box below shows how PvuII digests DNA molecules, with the arrowheads indicating strand cleavages sites.
Part A. (5 points) Which of the following terms best describes the ends of DNA molecules that result after digestion with PvuII?
a. Blunt-ended
b. Sticky-ended
c. Terminated
d. Replicated
Part B. (5 points) After performing the restriction digestion of your DNA sample with PvuII and running an agarose gel, you observe three bands on the gel: 50 bp, 450 bp, and 500 bp. How many recognition sites for PvuII are present in your original linear DNA sample? Write the answer in below.
Two
