**Guest Post** written by Leanna Matthews of Syracuse University                                       [Follow her on twitter @LeannaMatthews and check out her personal blog and website]

Every scientific journey begins with an idea.  These ideas can go one of two ways: 1) after countless trials and brainstorms, they actually work; 2) after countless trials and brainstorms, they don’t.  I think you can guess which one happens more often.

I came to the ORCAA lab as a visiting graduate student about a month ago with intentions of testing out some ideas.  Ideas that, when I posed them, were more like off-handed comments to my advisor rather than valid approaches to realistic data collection.  Let me back up a tiny bit…  I’m currently working on my PhD at Syracuse University with Dr. Susan Parks.  My interests are in pinniped behavior and physiology, and for my dissertation, I’m looking at the variation in male harbor seal mating behavior and its influence on reproductive success.  I’m also interested in the effects of shipping noise on harbor seals during the breeding season, but that’s another blog post for another time.

A few months ago, as I was furiously preparing for my PhD candidacy exam (one part proposal defense, one part general knowledge exam, all parts stressful), I met with Susan to discuss how I could get the data that I wanted for the project I was proposing.  I wanted underwater movements of harbor seals during the breeding season.  I wanted to map out male territories and really figure out how and where they were spending their time when they were below the surface.  Harbor seals, along with the majority of phocids, mate underwater.  Underwater behavior of any marine mammal is difficult to obtain.  We, as researchers, are limited in our visual observations to what happens above water.  The best method we have to tracking animals subsurface is tagging.  But tagging is expensive, time consuming, and logistically difficult – it typically involves getting a boat and a team of able-bodied persons, capturing the animal, and gluing a tag to its fur.  It’s doable, but not with solely my grad student resources.  Susan and I began spitting out other ideas:

“What about how they track fish, like a tiny PIT tag?”                                                          “Can we localize with a fish tag?”                                                                                              “They do it for salmon, no?”                                                                                                       “But how could we get the tag on the seal?”                                                                      “Some sort of remote attachment, so you wouldn’t have to capture them.”                               “Could we feed it to them?”                                                                                                       “That probably wouldn’t be a good idea…”                                                                                 “I guess we could just shoot it at them in a paintball.”

And there you have it, ladies and gentleman.  The mildly sarcastic comment that snowballed into a cross-country trip to Oregon and countless hours of researching glues and paintballs.

After a bit of post-meeting research, I came across some small high frequency acoustic transmitters (made by Vemco, pictured on the right) that are used to study fish movement.  They’re small, only about 16mm in length, and they emit 180 kHz signals about every 30 seconds.  These acoustic signals are picked up on receivers that are strategically moored in the study site.  By looking at differences in the times of arrival of specific signals, it’s possible to determine the location of the transmitter, i.e. the tag, i.e. the animal of interest.  Acoustic tags are great for looking at subsurface behavior because of how efficiently sound travels underwater (it’s much more efficient compared to air – you can read more about that here).  These tags seemed perfect!  They were small enough to fit in a paintball, they were the right kind of tag for studying underwater movement of individuals, and they emitted signals that were above the hearing threshold of harbor seals (and killer whales)*.

*Researchers have also used 69 kHz tags (instead of 180 kHz) to monitor fish populations.  The problem with these tags is that seals and sea lions can hear at 69 kHz.  Implanting a tag that emits a sound in the hearing range of the fish’s predator is basically attaching a dinner bell to the study organism.  When considering using acoustic tags on the seals, I wanted to make sure that they (and their predators) couldn’t hear the acoustic signal being emitted.  That way I could avoid any potential behavioral disturbance (or increased predation) caused by the sound coming from the tag.

I took my PhD candidacy exam, finished the semester, and packed my bags for Oregon, where ORCAA commander in chief Holger Klinck had agreed to help me test this weird tag attachment idea.

Current emotions: Excited.

Things we had to figure out:

• How do we get the tag inside the paintball?
• What kind of glue do we use? – something that doesn’t solidify inside the paintball, but cures almost immediately to the seal…hmmm does this product even exist?
• How do we close the paintball once the tag and the glue are inside?
• Will it actually stick to the seal? – we were going to need a real seal to test that one…

And thus began the Amazon binge-purchasing.  I bought glues.  Super glues.  Rapid cure super glues.  Super instant curing no drip super glues.  Veterinary grade surgical glues.  One-minute instant mix two-part epoxies.  Clear-dry power grip instant grab all-purpose interior adhesives.  I also bought some regular paintballs and some empty paintball shells.  And thanks to my paintballing sister, I already had the gun.

Current emotions: Overwhelmed – who knew there were so many options for adhesives?

The next step was to get the tag into the shell and fill it with glue.  This took a bit of finagling, but I finally did it!  I was so proud!  I made three types of paintball tags.  The first were regular paintballs that I emptied, stuffed with a tag, and filled with super glue (the green ones in the pictures below).  I sealed them with some glue and a sprinkle of baking soda.  It turns out that baking soda is an accelerant for cyanoacrylates (fancy name for super glue).  A tiny bit of baking soda and BOOM that super glue is SOLID.  The second type of tag ball was basically the same as the first, but I used the empty paintball shells (the clear ones in the pictures below).  Bonus – no emptying of paint required.  I was most proud of the third kind.  These were half filled with super glue and half filled with baking soda.  In theory, when it hit the seal, the tag would cure instantly to the fur of the animal because of the addition of the accelerant.

Current emotions:  Feelin’ creative and accomplished.

I’m going to keep this long post from becoming too long and just tell you that it didn’t work.  No tags stuck to anything.

Current emotions:  Disappointed, to say the least.

But this is science!  So what do we do?  We brainstorm more ideas!  And what do we do when those don’t work either??  We brainstorm even more ideas!  I went from my failed paintballs, to thinking about crossbows, tiny pistol crossbows, compound and recurve bows, drones (no one would buy me drones though…).  After lots of trial and error, with an emphasis on the error, I landed on the pistol crossbow.  It was small, manageable, and didn’t have too much power.  I crafted some bolts out of wooden dowels, foam floats, electrical tape, PVC end caps, fishing line, empty paintball shell halves (might as well use them if I’ve already got them, right?), and of course, duct tape.  With a little finesse and the right adhesive, I shot these homemade arrows out of my little crossbow and somehow got a tag to stick to my target.  I. was. shocked.  Did all of my brainstorming actually just pay off??

Current emotions: Chest-pounding, can-crushing, fire-breathing, unstoppable POWER.

At this point it had been a roller coaster of successes and failures, which I thought was going to end with my, what could only be described as, legendary tagging success.  However, after some preliminary field-testing, it was revealed that in order to make these tags work in the locations I wanted them to work, I would have to outfit the study area with an impractical number of receivers.  Had all the time and research and effort and crossbow target practice all been for naught?  Probably.

Current emotions:  Uggggghhhhh seriously??  COME ON.  I just got the tags to stick!

Back to the drawing board.  Conversations with Holger, conversations with Susan, and conversations with Holger and Susan at the same time led us all to the conclusion that the classic tagging approach would probably be the most logical way to go about getting my data.  Luckily, it’s looking like I’ll be able to collaborate with some other groups here in Oregon on a tagging trip that’s already planned for next year.  My sample size will be lower, it’s not exactly the data that I thought I was going to get, it’s not even in the same field site I thought I’d be working, but thus is life.  As a scientist, you can’t be married to a certain data collection method or even to a certain location.  You have to keep the big picture in mind – what were the original scientific questions/objectives?  If you’re still able to get at these major objectives, then you’re probably still doing alright.  Any data I can get to better understand the underwater mating behavior of these seals is beneficial for conservation and even just marine mammal biological knowledge in general.  There’s still so much we don’t know about the organisms that live in our oceans (even ones like seals that spend part of their lives on land), but slowly and surely, we’re picking away at the mysteries.

Current emotions: Back to being excited.  This scientific journey, though so far has been more madness then brilliance, is only beginning.  New pinniped adventures await!

-Leanna Matthews, PhD Candidate, Syracuse University