{"id":502,"date":"2015-09-19T04:03:03","date_gmt":"2015-09-19T04:03:03","guid":{"rendered":"http:\/\/blogs.oregonstate.edu\/bessummerinternship2015\/?p=502"},"modified":"2015-09-19T04:03:03","modified_gmt":"2015-09-19T04:03:03","slug":"week-9","status":"publish","type":"post","link":"https:\/\/dev.blogs.oregonstate.edu\/bessummerinternship2015\/2015\/09\/19\/week-9\/","title":{"rendered":"Week 9"},"content":{"rendered":"

 <\/p>\n

During this internship, we extracted<\/a> a lot of DNA from various samples. In week 9, I was able to take some pictures of how we obtained those samples.<\/p>\n

 <\/p>\n

The first step is to put a large cube of the agar into a flask containing a broth…<\/p>\n

\"11949796_763375860438512_764387505_n\"<\/a><\/p>\n

Then the flasks are put onto a shaker that spins the broth and agar around. We left them like this for about a week.<\/p>\n

\"12007022_774658045976960_1405092172_n\"<\/a><\/p>\n

 <\/p>\n

 <\/p>\n

We waited until enough growth had occurred to harvest it…<\/p>\n

\"11910861_763375793771852_1220529627_n<\/a><\/p>\n

\"11938128_763375840438514_72735303_n\"<\/a><\/p>\n

Then we strained the flasks, collecting the tissue in autoclaved filter paper.<\/p>\n

\"12033439_774658072643624_1758687518_n\"<\/a><\/p>\n

We then pressed the excess water out of the tissue. It kind of looked like (not appetizing) cheese!<\/p>\n

\"12026418_774658132643618_2141169252_n\"<\/a><\/p>\n

It got put into tubes that were then sealed with kim wipes and parafilm.<\/p>\n

\"12033781_774658149310283_1573601026_n\"<\/a><\/p>\n

\"12030820_774658162643615_1900402542_n\"<\/a><\/p>\n

 <\/p>\n

Into the freezer they went! Afterwards they were lyophilized and then used for DNA extractions.<\/p>\n

\"12016483_774658209310277_1718155520_n\"<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

  During this internship, we extracted a lot of DNA from various samples. In week 9, I was able to take some pictures of how we obtained those samples.   The first step is to put a large cube of the agar into a flask containing a broth… Then the flasks are put onto a … Continue reading Week 9<\/span><\/a><\/p>\n","protected":false},"author":6974,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-502","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"_links":{"self":[{"href":"https:\/\/dev.blogs.oregonstate.edu\/bessummerinternship2015\/wp-json\/wp\/v2\/posts\/502","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/dev.blogs.oregonstate.edu\/bessummerinternship2015\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/dev.blogs.oregonstate.edu\/bessummerinternship2015\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/dev.blogs.oregonstate.edu\/bessummerinternship2015\/wp-json\/wp\/v2\/users\/6974"}],"replies":[{"embeddable":true,"href":"https:\/\/dev.blogs.oregonstate.edu\/bessummerinternship2015\/wp-json\/wp\/v2\/comments?post=502"}],"version-history":[{"count":1,"href":"https:\/\/dev.blogs.oregonstate.edu\/bessummerinternship2015\/wp-json\/wp\/v2\/posts\/502\/revisions"}],"predecessor-version":[{"id":512,"href":"https:\/\/dev.blogs.oregonstate.edu\/bessummerinternship2015\/wp-json\/wp\/v2\/posts\/502\/revisions\/512"}],"wp:attachment":[{"href":"https:\/\/dev.blogs.oregonstate.edu\/bessummerinternship2015\/wp-json\/wp\/v2\/media?parent=502"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/dev.blogs.oregonstate.edu\/bessummerinternship2015\/wp-json\/wp\/v2\/categories?post=502"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/dev.blogs.oregonstate.edu\/bessummerinternship2015\/wp-json\/wp\/v2\/tags?post=502"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}