{"id":104,"date":"2015-06-28T01:30:59","date_gmt":"2015-06-28T01:30:59","guid":{"rendered":"http:\/\/blogs.oregonstate.edu\/bessummerinternship2015\/?p=104"},"modified":"2015-07-06T22:12:33","modified_gmt":"2015-07-06T22:12:33","slug":"week-1-pcr","status":"publish","type":"post","link":"https:\/\/dev.blogs.oregonstate.edu\/bessummerinternship2015\/2015\/06\/28\/week-1-pcr\/","title":{"rendered":"Week 1- PCR!"},"content":{"rendered":"

Another project that I was able to work on during my first week was PCR using\u00a0DNA from different samples of ergot, a fungi that grows on\u00a0plants such as blue grass and wheat. If ergot is ingested it can lead to vasoconstriction and can also cause hallucinations.<\/p>\n

\"11647170_737365243039574_1030950951_n\"<\/a>
Different samples of ergot DNA<\/figcaption><\/figure>\n

To perform the PCR, we had to combine primers, water, and master mix into each capsule along with the different DNAs. The master mix is a bright green color because it contains ingredients that help it sink into the gel later in the PCR.\u00a0\"11652138_737365056372926_958455970_n\"<\/a>When pipetting all of the primers, master mix, and DNA together, you have to be very careful to not contaminate any of the capsules and to not mix the DNAs together. It can be tricky to remember what you put where with so many different tiny tubes!\"11124892_737365036372928_2087562588_n\"<\/p>\n

\"11269517_737379936371438_956346387_n\"After all of the solutions were combined, the capsules were centrifuged and then put into the PCR machine. This machine changes the temperature for specific amounts of time so that the primers and master mix can work to replicate certain sections of the DNA over and over.\u00a0\"11667022_737364883039610_945274925_n\"After the PCR machine was done, we created a gel using agarose. We put combs into the gel to make tiny wells. When the gel had firmed up, it was placed into the electrophoresis machine.\u00a0\"11650707_737364733039625_1993304658_n\"<\/a>Each DNA solution had to be carefully transferred into the wells of the gel using a pipette.\"11651078_737364706372961_599055866_n\"<\/a>The voltage of the electrophoresis machine was turned to 120 V. DNA has a negative charge so it travels towards the positively charged end of the machine.\u00a0\"11647195_737364653039633_1838097742_n\"<\/a>The agarose gel created a matrix for the DNA to move through which made the smaller pieces move farther than the larger pieces.<\/p>\n

\"11667931_737364613039637_1274555749_n\"<\/a>
Faint yellow and blue lines showed where the DNA traveled<\/figcaption><\/figure>\n

The sheet of agarose was carefully placed into a GelDoc-It Imager.\"11647335_737364559706309_1299795515_n\"<\/a>The window of the imager allows you to see the gel under UV light.
\n\"11668169_737364556372976_1120440093_n\"<\/a>The imager also allows you to record a computer image of the picture to examine for later use.\u00a0\"11124881_737364546372977_997457231_n\"<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

Another project that I was able to work on during my first week was PCR using\u00a0DNA from different samples of ergot, a fungi that grows on\u00a0plants such as blue grass and wheat. If ergot is ingested it can lead to vasoconstriction and can also cause hallucinations. To perform the PCR, we had to combine primers, … Continue reading Week 1- PCR!<\/span><\/a><\/p>\n","protected":false},"author":6974,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-104","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"_links":{"self":[{"href":"https:\/\/dev.blogs.oregonstate.edu\/bessummerinternship2015\/wp-json\/wp\/v2\/posts\/104","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/dev.blogs.oregonstate.edu\/bessummerinternship2015\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/dev.blogs.oregonstate.edu\/bessummerinternship2015\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/dev.blogs.oregonstate.edu\/bessummerinternship2015\/wp-json\/wp\/v2\/users\/6974"}],"replies":[{"embeddable":true,"href":"https:\/\/dev.blogs.oregonstate.edu\/bessummerinternship2015\/wp-json\/wp\/v2\/comments?post=104"}],"version-history":[{"count":5,"href":"https:\/\/dev.blogs.oregonstate.edu\/bessummerinternship2015\/wp-json\/wp\/v2\/posts\/104\/revisions"}],"predecessor-version":[{"id":210,"href":"https:\/\/dev.blogs.oregonstate.edu\/bessummerinternship2015\/wp-json\/wp\/v2\/posts\/104\/revisions\/210"}],"wp:attachment":[{"href":"https:\/\/dev.blogs.oregonstate.edu\/bessummerinternship2015\/wp-json\/wp\/v2\/media?parent=104"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/dev.blogs.oregonstate.edu\/bessummerinternship2015\/wp-json\/wp\/v2\/categories?post=104"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/dev.blogs.oregonstate.edu\/bessummerinternship2015\/wp-json\/wp\/v2\/tags?post=104"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}