During this internship, we extracted a lot of DNA from various samples. In week 9, I was able to take some pictures of how we obtained those samples.
The first step is to put a large cube of the agar into a flask containing a broth…
Then the flasks are put onto a shaker that spins the broth and agar around. We left them like this for about a week.
We waited until enough growth had occurred to harvest it…
Then we strained the flasks, collecting the tissue in autoclaved filter paper.
We then pressed the excess water out of the tissue. It kind of looked like (not appetizing) cheese!
It got put into tubes that were then sealed with kim wipes and parafilm.
Into the freezer they went! Afterwards they were lyophilized and then used for DNA extractions.
